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housekeeping gene gapdh  (Proteintech)


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    Structured Review

    Proteintech housekeeping gene gapdh
    Housekeeping Gene Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/housekeeping+gene+gapdh/GAPDH+Monoclonal+antibody/pmc12596845-75-33-36
    Average 98 stars, based on 10565 article reviews
    housekeeping gene gapdh - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Mesenchymal stem cell-secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation.
    Article Snippet: Many chemotherapeutics exacerbate testicular injuries, including oligozoospermia, teratozoospermia and irreversible azoospermia, and lead to infertility and inferior quality of life (Gandini et al., 2006; Meistrich, 2009).. Sperm cryopreservation seems to be the only es‐ tablished fertility preservation method in males (Martinez, 2017).. When semen cryopreservation is not possible for those patients like pre‐pubertal children, many other experimental therapies to protect gonads should be explored.

    Blocking Assay:

    Article Title: Mesenchymal stem cell-secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation.
    Article Snippet: Many chemotherapeutics exacerbate testicular injuries, including oligozoospermia, teratozoospermia and irreversible azoospermia, and lead to infertility and inferior quality of life (Gandini et al., 2006; Meistrich, 2009).. Sperm cryopreservation seems to be the only es‐ tablished fertility preservation method in males (Martinez, 2017).. When semen cryopreservation is not possible for those patients like pre‐pubertal children, many other experimental therapies to protect gonads should be explored.

    Western Blot:

    Article Title: Rapamycin and Chloroquine Modulate Insulin Resistance and Hepatic Steatosis in a High-Fat/High-Cholesterol Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease Mouse Model
    Article Snippet: Primary antibody incubation was conducted overnight at 4°C using antibodies against FASN, PLIN2, LC3, P62, and α-SMA (Proteintech, 1:5000 dilution), followed by 1-hour incubation with HRP-conjugated secondary antibody (1:5000 dilution) at room temperature (The specific genetic information can be found in Supplementary Information Tables 1 and 2 ). .. Protein bands were visualized using an Immobilon immunoblotting detection system (Millipore) combined with a ChemiDoc imaging system (Bio-Rad Laboratories, Inc)., with band intensity quantified via Image Lab 6.1 software and normalized to the housekeeping gene GAPDH (Proteintech 60004-1-Ig). .. Total RNA was isolated from liver tissues or cultured cells using TransZol Up Reagent (TransGen Biotech ET111-01), reverse-transcribed to cDNA using the EasyScript One-Step gDNA Removal Kit (TransGen Biotech AE311-04), and amplified with TransStart Top Green qPCR Super Mix (TransGen Biotech AQ131-04).

    Imaging:

    Article Title: Rapamycin and Chloroquine Modulate Insulin Resistance and Hepatic Steatosis in a High-Fat/High-Cholesterol Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease Mouse Model
    Article Snippet: Primary antibody incubation was conducted overnight at 4°C using antibodies against FASN, PLIN2, LC3, P62, and α-SMA (Proteintech, 1:5000 dilution), followed by 1-hour incubation with HRP-conjugated secondary antibody (1:5000 dilution) at room temperature (The specific genetic information can be found in Supplementary Information Tables 1 and 2 ). .. Protein bands were visualized using an Immobilon immunoblotting detection system (Millipore) combined with a ChemiDoc imaging system (Bio-Rad Laboratories, Inc)., with band intensity quantified via Image Lab 6.1 software and normalized to the housekeeping gene GAPDH (Proteintech 60004-1-Ig). .. Total RNA was isolated from liver tissues or cultured cells using TransZol Up Reagent (TransGen Biotech ET111-01), reverse-transcribed to cDNA using the EasyScript One-Step gDNA Removal Kit (TransGen Biotech AE311-04), and amplified with TransStart Top Green qPCR Super Mix (TransGen Biotech AQ131-04).

    Software:

    Article Title: Rapamycin and Chloroquine Modulate Insulin Resistance and Hepatic Steatosis in a High-Fat/High-Cholesterol Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease Mouse Model
    Article Snippet: Primary antibody incubation was conducted overnight at 4°C using antibodies against FASN, PLIN2, LC3, P62, and α-SMA (Proteintech, 1:5000 dilution), followed by 1-hour incubation with HRP-conjugated secondary antibody (1:5000 dilution) at room temperature (The specific genetic information can be found in Supplementary Information Tables 1 and 2 ). .. Protein bands were visualized using an Immobilon immunoblotting detection system (Millipore) combined with a ChemiDoc imaging system (Bio-Rad Laboratories, Inc)., with band intensity quantified via Image Lab 6.1 software and normalized to the housekeeping gene GAPDH (Proteintech 60004-1-Ig). .. Total RNA was isolated from liver tissues or cultured cells using TransZol Up Reagent (TransGen Biotech ET111-01), reverse-transcribed to cDNA using the EasyScript One-Step gDNA Removal Kit (TransGen Biotech AE311-04), and amplified with TransStart Top Green qPCR Super Mix (TransGen Biotech AQ131-04).



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